Cochranella buenaventura sp. nov. is described from the southern foothills of the Cordillera Occidental, Andes of Ecuador. The new species inhabits the Seasonal Foothill Evergreen forests of the province of El Oro, in the West Ecuadorian biogeographic region. This species is distinguished from other species of glassfrogs by having a truncate snout in dorsal and lateral views, reduced webbing between fingers, absence of guanophores on the digestive visceral peritonea, but present on the renal capsule, absence of humeral spine, green dorsum in life with scattered pale yellow spots, bright yellow hands and feet discs, and moderate body size (20.9-22.4 mm snout-vent length in adult males).