A survey of biology departments in 1990 led to publication of a de facto curriculum for the training of undergraduate biology majors. Knowledge of the biological sciences has changed considerably since then, and the present study attempts to find out whether or not undergraduate requirements have changed as a result. In fact, little has changed, although the molecular areas of biology are more likely to be required now than they were in 1990. In the absence of a national accrediting body for biology, questions remain about whether there needs to be a standardized curriculum and, if so, what it should contain. This study includes a look at what courses are offered by undergraduate departments, what courses are required for a biology degree, and what content is covered in the introductory course sequence. Suggestions for an updated curriculum are also provided.