Fringed myotis (Myotis thysanodes) is considered a bat species of conservation concern in Colorado and in western North America. The purpose of our research was to describe the geographic and elevational distribution of fringed myotis in Colorado and consider the potential influence of the Southern Rocky Mountains on the distribution of this species. We documented, mapped, and analyzed 729 Colorado capture and occurrence records for this species, and used 546 records in a 2-way ANOVA comparing elevational distribution among sex and reproductive classes. Mean elevation of occurrence was significantly different among reproductive classes (F = 7.03, P = 0.0010) but not between sex classes (F = 0.10, P = 0.7578). These elevation results support the hypothesis that fringed myotis tend not to occur at higher elevations in the Southern Rockies. Mapped occurrence records suggest that fringed myotis exhibits a bifurcated geographic distribution, with separate populations occurring in a band along the Colorado Front Range and in western Colorado. These data suggest that the high peaks, mountain parks, and valleys of the Southern Rocky Mountains act as a set of dispersal barriers, and that movement by fringed myotis from east to west, and vice versa, across the Colorado Rockies is not a regular occurence. Finally, populations occurring in southeastern Colorado and in a band along the Colorado Front Range may potentially be at risk from increasing urbanization, changes in surface water resource availability, and exposure to diseases, such as white-nose syndrome.