The increased incidence of glyphosate-resistant weeds has led to an exponential increase in the use of glufosinate on glufosinate-resistant corn, cotton, and soybean crops. Field experiments were conducted in 2021 and 2022 to evaluate peanut response to glufosinate at 25 and 60 d after planting, corresponding to vegetative (V3) and reproductive (R4) growth stages, at 1.2, 4.7, 18.9, 75.5, and 302 g ai ha^-1 representing 1/514 to 1/2 of the labeled rate of 604 g ha^-1. Peanut injury and canopy and yield reductions from glufosinate were <10% when applied at 1.2, 4.7, and 18.9 g ha^-1. However, at 75.5 and 302 g ha^-1 peanut injury ranged from 24% to 72% at the V3 exposure timing and 33% to 54% at the R4 exposure timing. Similarly, glufosinate applied at 75.5 and 302 g ha^-1 reduced peanut canopy width by 10% to 23% at the V3 exposure timing and by 43% to 57% at the R4 exposure timing. Averaged across exposure timing, peanut yield was reduced by 15% and 61% when glufosinate was applied at 75.5 and 302 g ha^-1, respectively. Averaged across rates, peanut yield reduction was 18% at the V3 exposure timing, with glufosinate at 298 g ha^-1 required to cause an estimated 50% reduction in yield. Peanut yield was reduced by 20% when glufosinate was applied at the R3 peanut growth stage, whereas glufosinate applied at 243 g ha^-1 caused an estimated 50% reduction in yield. There was no difference in normalized difference vegetative index (NDVI) values between untreated plants and peanut exposed to glufosinate at 1.2, 4.7, and 18.9 g ha^-1. However, peanut exposed to glufosinate at 75.5 and 302 g ha^-1 was distinguished from untreated plants with lower NDVI values. Based on the Pearson correlation coefficient, the best timing for assessing potential yield reduction based on injury was between 2 and 4 wk after treatment.

Nomenclature: Glufosinate; peanut, Arachis hypogaea L.