A native strain of Heterorhabditis indica Poinar, Karunakar & David nematode from Guasave, Sinaloa, Mexico, was identified by sequencing internal transcribed spacer regions of ribosomal DNA (rDNA). Assays against immature sweetpotato whitefly, Bemisia tabaci (Gennadius), used four strains of nematodes: Heterorhabditis bacteriophora Poinar Guatemala strain, H. bacteriophora Chiapas strain, a commercial product Steinernema feltiae (Filipjev) (Koppert®), and the H. indica native strain from Sinaloa. All strains at concentrations of 1,000, 750, 500, and 250 infective juveniles per milliliter, without significant differences among them, killed 70 to 95% of B. tabaci biotype B nymphs on leaves of tomato, Solanum lycopersicum (Mill), under laboratory conditions. This study is the first report of a native H. indica strain in Mexico, which is proposed as a biological control agent against B. tabaci.