Many small mammal species experience population declines following prescribed fire, presumably resulting from increased predation due to lack of cover. However, Peromyscus gossypinus (Cotton Mouse) typically shows a neutral or positive population response following fire. Because they typically spend diurnal hours in below-ground refuges, Cotton Mice may be less susceptible to predation following fire than other small mammals. We examined the effects of prescribed fire and exclusion of mammalian predators on selection of daytime refuges by Cotton Mice. We located daytime refuges of 12 radiotagged Cotton Mice in a fenced mesomammal-predator (hereafter, mesopredator) exclosure (23 refuge locations) and 9 Cotton Mice in an adjacent unfenced control plot (13 refuge locations) for one month prior to and one month after a prescribed fire in winter 2007. Refuge locations included Gopherus polyphemus (Gopher Tortoise) burrows (27.8%), other ground holes (44.4%), stump holes (25.0%), and holes at the base of trees (2.8%). Fire had little effect on refuge selection, likely because Cotton Mice primarily used below-ground refuges, which allowed them to avoid the direct effects of fire and predation following fire. Structure near the refuge, including burrows, stumps, and coarse woody debris, was important in selection of daytime refuges and was particularly important in the presence of mesopredators.