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29 October 2010 Transcriptome Characterization Uncovers the Molecular Response of Hematopoietic Cells to Ionizing Radiation
Alexandre Pawlik, Olivier Alibert, Sylvain Baulande, Pierre Vaigot, Diana Tronik-Le Roux
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Abstract

Ionizing radiation causes rapid and acute suppression of hematopoietic cells that manifests as the hematopoietic syndrome. However, the roles of molecules and regulatory pathways induced in vivo by irradiation of different hematopoietic cells have not been completely elaborated. Using a strategy that combined different microarray bioinformatics tools, we identified gene networks that might be involved in the early response of hematopoietic cells radiation response in vivo. The grouping of similar time-ordered gene expression profiles using quality threshold clustering enabled the successful identification of common binding sites for 56 transcription factors that may be involved in the regulation of the early radiation response. We also identified novel genes that are responsive to the transformation-related protein 53; all of these genes were biologically validated in p53-transgenic null mice. Extension of the analysis to purified bone marrow cells including highly purified long-term hematopoietic stem cells, combined with functional classification, provided evidence of gene expression modifications that were largely unknown in this primitive population. Our methodology proved particularly useful for analyzing the transcriptional regulation of the complex ionizing radiation response of hematopoietic cells. Our data may help to elucidate the molecular mechanisms involved in tissue radiosensitivity and to identify potential targets for improving treatment in radiation emergencies.

Alexandre Pawlik, Olivier Alibert, Sylvain Baulande, Pierre Vaigot, and Diana Tronik-Le Roux "Transcriptome Characterization Uncovers the Molecular Response of Hematopoietic Cells to Ionizing Radiation," Radiation Research 175(1), 66-82, (29 October 2010). https://doi.org/10.1667/RR2282.1
Received: 4 April 2010; Accepted: 1 August 2010; Published: 29 October 2010
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