OPTIMIZED NESTED POLYMERASE CHAIN REACTION FOR ANTEMORTEM DETECTION OF MYCOBACTERIA IN AMAZON PARROTS (AMAZONA AESTIVA) AND ORANGE-WINGED AMAZONS (AMAZONA AMAZONICA)

Arianne Costa Baquião; Janaina Oliveira Luna; Aziz Orro Medina; Luiz Francisco Sanfilippo; Maria Jacinta de Faria; Manuel Armando Azevedo dos Santos

doi:10.1638/2013-0019R1.1

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Arianne Costa Baquião, Janaina Oliveira Luna, Aziz Orro Medina, Luiz Francisco Sanfilippo, Maria Jacinta de Faria, Manuel Armando Azevedo dos Santos

Abstract

The objectives of this study were to optimize nested polymerase chain reaction (PCR) for Mycobacterium avium complex and Mycobacterium tuberculosis complex and apply them on samples from parrots. Results were negative for the presence of these Mycobacterium in the samples, and nested PCR was specific, faster, and more sensitive than other tests, thereby justifying its use in antemortem diagnosis.

American Association of Zoo Veterinarians

Citation Download Citation

Arianne Costa Baquião, Janaina Oliveira Luna, Aziz Orro Medina, Luiz Francisco Sanfilippo, Maria Jacinta de Faria, and Manuel Armando Azevedo dos Santos "OPTIMIZED NESTED POLYMERASE CHAIN REACTION FOR ANTEMORTEM DETECTION OF MYCOBACTERIA IN AMAZON PARROTS (AMAZONA AESTIVA) AND ORANGE-WINGED AMAZONS (AMAZONA AMAZONICA)," Journal of Zoo and Wildlife Medicine 45(1), 161-164, (1 March 2014). https://doi.org/10.1638/2013-0019R1.1

Received: 17 February 2013; Published: 1 March 2014

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