The National Zoological Garden plays a vital role in conservation of reptiles in Sri Lanka. Since parasitic infestations of captive reptiles can impact their health, a survey for intestinal parasites and ectoparasites was conducted on 19 selected reptilian species (14 snakes, four chelonians, and one crocodilian) housed at the National Zoological Garden, Sri Lanka. Of the reptiles screened, 62% (N = 139) were infected with parasites; 66% and 24% exclusively harbored intestinal and ecto parasites, respectively, while 10% carried both types of parasites. Three ticks (Ixodidae), two adult cestodes, plerocercoid larvae, and four nematode species were recovered during this survey. Three types of nematode ova and a single type of digenian ova, protozoan cysts, L3 nematode larvae, and a protozoan were detected in the feces. In this first systematic survey of reptilian parasites in Sri Lanka, four new host–parasite records are documented.