Viral hemorrhagic septicemia virus (VHSV) infects wild and hatchery fish in Europe, Japan, and the Great Lakes and Pacific regions of North America. The virus was associated with a large die-off of yellow perch, Perca flavescens, in Lake Erie in 2006. To determine the infection pattern of VHSV, we sampled yellow perch during the spring, summer, and fall of 2007 and 2008 in the central basin of Lake Erie during routine sampling by the Ohio Division of Wildlife with bottom trawls in nearshore, mid-depth, and offshore locations near the Chagrin River. The Ohio Department of Agriculture's Diagnostic Laboratories and the U.S. Fish and Wildlife Service's La Crosse Fish Health Center tested for VHSV from homogenized samples obtained from yellow perch kidney, spleen, and brain. At each lake sample location, we also measured temperature, dissolved oxygen, and conductivity. In both years, we found yellow perch infected with VHSV during a threeweek period starting in the last week of spawning to early June. A high proportion of adult male and female yellow perch tested positive for VHSV during the infection period in our sample population. Infection appeared to be associated with temperatures between 12 and 18 °C and with significantly higher yellow perch densities during spawning. No large mortalities of yellow perch were observed during the VHSV infection period in 2007 and 2008.