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1 December 2010 Diagnostic Utility of Egg Yolk for the Detection of Avian Metapneumovirus Antibodies in Laying Hens
Kang-Seuk Choi, Eun-Kyoung Lee, Woo-Jin Jeon, Mi-Ja Park, Yae-Na Yoo, Jun-Hun Kwon
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Abstract

Surveillance and diagnosis of avian metapneumovirus (AMPV) infection typically involve measurement of serum antibodies. In the current study, eggs instead of serum samples were used for the detection of AMPV antibodies in egg-laying chicken hens by enzyme-linked immunosorbent assay (ELISA). AMPV-free commercial layer hens were experimentally challenged with AMPV strain SC1509 through intravenous or oculonasal administration. Antibody levels were determined by ELISA. AMPV antibodies were detected in egg yolks from challenged hens by 7 days postinoculation (dpi), with the peak titer at 16 dpi. Antibody levels in eggs laid at 28 dpi correlated well (r  =  0.93) with sera taken 28 dpi from the same hens. In a field trial of the yolk ELISA, six broiler breeder farms were surveyed, and all tested positive for AMPV antibodies in hen eggs, although positivity varied from farm to farm. Abnormal discolored eggs collected from outbreak farms had significantly higher titers of AMPV yolk antibodies than normal eggs from the same farm, unlike clinically healthy farms, where normal and abnormal eggs had similar antibody titers. These results indicate that diagnosis of AMPV infection by yolk ELISA to detect anti-AMPV antibodies may be a suitable alternative to serologic testing.

Kang-Seuk Choi, Eun-Kyoung Lee, Woo-Jin Jeon, Mi-Ja Park, Yae-Na Yoo, and Jun-Hun Kwon "Diagnostic Utility of Egg Yolk for the Detection of Avian Metapneumovirus Antibodies in Laying Hens," Avian Diseases 54(4), 1230-1236, (1 December 2010). https://doi.org/10.1637/9382-042710-Reg.1
Received: 30 April 2010; Accepted: 1 August 2010; Published: 1 December 2010
KEYWORDS
avian metapneumovirus
diagnosis
yolk antibody
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