Great Smoky Mountains National Park is an area known for its incredible species diversity. This study was conducted in order to determine the vertical distribution of lichen growth forms and overall lichen species richness on selected host-tree species in the Park. Specifically, lichens were collected from Tilia americana var. heterophylla (basswood), Abies fraseri (Fraser fir), Liquidambar styraciflua (sweet gum), and Fraxinus americana (white ash) at different canopy heights. A total of eight trees (two for each species) were sampled using the double-rope climbing technique. Overall, our results indicate that both host-tree species and canopy height influence lichen species richness and distribution of growth forms. Three lichen species are reported as candidates for new records in the Park: Gyalecta flotowii Korber, Lecanora miculata Ach., and Pyrenula pseudobufonia (Rehm) R.C. Harris.