Atmospheric nitrogen (N) deposition may affect carbon (C) sequestration in terrestrial ecosystem. The main objective of this paper was to test the hypothesis that N addition would increase CO₂ emission in the N limited meadow steppe in Inner Mongolia, China. Response of CO₂ fluxes to simulated N deposition was studied in the growing season of 2008 and 2009 by static chamber and gas chromatograph techniques. Parallel to the flux measurements, soil temperature, soil moisture, TOC, DOC, soil NH₄ and NO₃^- were measured at the same time. The results indicated that two-year N additions had no significant effect on NH₄ , but slightly increased NO₃^- in the later period. The HN treatment tended to increase CO₂ fluxes in the two years, and LN treatment tended to decrease CO₂ fluxes in 2008, and shifted to increase CO₂ fluxes in later growing season of 2009. N addition significantly increased the aboveground biomass and root biomass. The correlation between CO₂ fluxes and moisture or temperature factors did not significantly change due to N addition, but N addition enhanced the moisture sensitivity of CO₂ fluxes as well as the temperature sensitivity of CO₂ fluxes. These results suggest that the increasing ammonium N deposition would be likely to stimulate CO₂ fluxes in the meadow steppe of Inner Mongolia, China.