We present the results of a chromosomal study (C-, Ag-NOR-, endonuclease digestion, DAPI and CMA₃ banding) carried out on four Tunisian species of amphibians: Bufo mauritanicus, B. viridis, Rana saharica and Discoglossus pictus auritus. For comparative purposes, our cytogenetic analysis was also extended to Moroccan specimens of B. mauritanicus and D. pictus scovazzi. Tunisian specimens of B. viridis display chromosome and chromatin characters very similar to those found in other specimens, both North African and Euro-Asian. By contrast, chromosomes of Tunisian specimens of B. mauritanicus differed from the Moroccan specimens in presenting a different Q-banding phenotype. Tunisian specimens of R. saharica displayed the plesiomorphic Pelophylax karyotype of 2n = 26 biarmed chromosomes and NORs on the eleventh pair. The Tunisian specimens of D. pictus auritus had the typical D. pictus 2n = 28 karyotype with the 13th pair of telocentric elements, in contrast to the Spanish auritus specimens that display the chromosomes of this pair shaped as metacentric. Similarities and differences of the four studied Tunisians anuran taxa are discussed with references to published data on their relatives.