Long Point, Ontario is an important stopover site for a variety of migrating birds and it may also serve migrating bats. While bats often have been caught during mist netting for birds at Long Point, there have been no quantitative studies of migrating (or resident) bats at this location. We monitored bat activity at Long Point using mist-net captures and recordings of echolocation calls in June and August 2006. Bat activity was significantly higher in August than in June, suggesting a peak in bat activity coincident with when migration would be expected to occur. Lasionycteris noctivagans was the only species known from Ontario not present at Long Point in June, but it was the second most abundant bat in August. An increase in L. noctivagans and Lasiurus cinereus abundance between June and August, suggests that Long Point serves as a migratory flyway for both species. Feeding activity of L. noctivagans increased towards the end of August, suggesting that it and not others use Long Point as a stopover and refuelling site. Mitochondrial haplotypes of Myotis lucifugus were more diverse at Long Point than at a swarming site in Ontario, indicating that Long Point is important for bats, regardless of its function as a stopover site during migration.