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Annales Zoologici 60(4):639-646. 2010
https://doi.org/10.3161/000345410X550508

Assessment of Intraspecific mtDNA Variability of the Water Frog Pelophylax saharicus in Eastern North Africa
No Access
Amor Nabil
Farjallah Sarra
Merella Paolo
Ben-Yacoub Slim
Said Khaled

Received: May 5, 2010; Accepted: November 6, 2010

Author Affiliations

Amor Nabil

Research Unit: Genetics, Biodiversity and Bio-Resources Development, Biotechnology Institute of Monastir, 5000 Monastir, Tunisia

Author for correspondence:

Farjallah Sarra

Research Unit: Genetics, Biodiversity and Bio-Resources Development, Biotechnology Institute of Monastir, 5000 Monastir, Tunisia

Merella Paolo

Sezione di Parassitologia e Malattie Parassitarie Dipartimento di Biologia Animale, Università degli Studi di Sassari, Sardaigne, Italy

Ben-Yacoub Slim

Laboratory of Terrestrial and Aquatic Systems Ecology. University BADJI Mokhtar Annaba, Algeria

Said Khaled

Research Unit: Genetics, Biodiversity and Bio-Resources Development, Biotechnology Institute of Monastir, 5000 Monastir, Tunisia

Copyright & Usage

© Fundacja Natura optima dux

Abstract.

The water frog Pelophylax saharicus is the most widespread anuran in North Africa and thus subjected to widely differing environmental conditions in different parts of its distribution range. In this paper we examined genetic structure of species 15 populations across Tunisia and eastern Algeria. We evaluated a potential role of mountain ranges as significant barriers to gene flow, using a partial sequences of the mitochondrial cytochrome oxidase I gene (COI). Twenty haplotypes were recorded in a total sample of 38 individuals, whereas, an overall low genetic variation of 0.4% was observed. AMOVA revealed no significant genetic structuring related to the 4 groups across the studied area. Unimodal mismatch distributions and significantly negative values of Fu's Fs and Tajima's D statistics support a recent expansion of populations from a smaller founder population as the most plausible explanation of the observed significant deviations from neutrality in the North-East African green frog populations.


REFERENCES

Amor, N., Aprea, G., Chatti, N., Farjallah, S., Odierna, G. and K. Said. 2007. Karyologieal analysis of four Tunisia species of Anura (Amphibia). African Zoology, 42: 268278. BioOne, Google Scholar
Amor, N., Kalboussi, M. and K. Said. 2009a. Conservation status of amphibians in Tunisia. In: Amphibian Biology, Volume 8C: the status of Amphibian Decline. Heatwole, H. (Editor). Surrey Beatty & Sons, Chipping Norton, 327 pp. Google Scholar
Amor, N., Farjallah, S. and K. Said. 2009b. Morphometric variation in the Tunisian green Frog, Rana saharica (Anura: Ranidae). African Zoology, 43(2): 194203. Google Scholar
Arano, B., Lorente, G. A., Montori, A., Buckley, D. and P. Herrero. 1998. Diversification in north-west African water frogs: molecular and morphological evidence. Herpetological Journal, 8(1): 5764. Google Scholar
Boulenger, G. A. 1891. Catalogue of the reptiles and batrachians of Barbary (Morocco, Algeria, Tunisia) based chiefly upon the notes and collections made in 1880–1884 by M. Fernand Lataste. Transactions of the Zoological Society of London, 13: 93164. Crossref, Google Scholar
Buckley, D., Arano, B., Herrero, P., Llorente, G. and M. Esteban. 1994. Moroccan Water Frogs vs. R. perezi: allozyme studies show their differences. Zoologica Poloniae, 39(3–4): 377385. Google Scholar
Buckley, D., Arano, B., Herrero, P. and G. Llorente. 1996. Population structure of Moroccan Water Frogs: genetic cohesion despite a fragmented distribution. Journal of Zoological Systematics and Evolutionary Research, 34(3): 173179. Crossref, Google Scholar
Clement, M, Posada, D. and K. A. Crandall. 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9: 16571660. Crossref, Google Scholar
Desjardins, P. and R. Morais. 1990. Sequence and gene organization of the chicken mitochondrial gene. A novel gene order in higher vertebrates. Journal of Molecular Biology, 212: 599634. Crossref, Google Scholar
De Jong, H. 1998. In search of historical biogeographic patterns in the western Mediterranean terrestrial fauna. Biological Journal of the Linnean Society, 65: 99164. Crossref, Google Scholar
Duellman, W. E. and L. Trueb. 1986. Biology of Amphibians, McGraw-Hill Book Company., New York 670 pp. Google Scholar
Excoffier, L., Laval, G. and S. Schneider. 2005. Arlequin ver. 3.1: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1: 4750. Crossref, Google Scholar
Fu, Y. X. 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147: 91592. Google Scholar
Funk, W. C., Blouin, M. S, Corn, P. S., Maxell, B. A., Pilliod, D. S., Amish, S. and F. W. Allendorf. 2005. Population structure of Columbia spotted frogs (Rana luteiventris) is strongly affected by the landscape. Molecular Ecology, 14: 483496. Crossref, Google Scholar
Gueddari, M., Monnin, C., Perret, D., Fritz, B. and Y. Tardy. 1983. Geochemistry of brines of the Chott el Djerid in southern Tunisia: application of Pitzer's equations, Chemical Geology, 39: 165178. Crossref, Google Scholar
Guiller, A. and L. Madec. 2010. Historical biogeography of the land snail Cornu aspersum: a new scenario inferred from haplotype distribution in the Western Mediterranean basin. BMC Evolutionary Biology, 10: 18. Crossref, Google Scholar
Harris, D. J., Batista, V. and M. A. Carretero. 2003. Diversity of 12S mitochondrial DNA sequences in Iberian and north-west African water frogs across predicted geographic barriers. Herpetozoa, 16(1/2): 8183. Google Scholar
Harpending, H., Batzer, M., Gurven, M., Jorde, L., Rogers, A. and S. Sherry. 1998. Genetic traces of ancient demography. Proceedings of the National Academy of Sciences USA, 95: 19611967. Crossref, Google Scholar
Kumar, S., Tamura, K. and N. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics, 5: 150163. Crossref, Google Scholar
Mayet, V. 1903. Catalogue of Reptiles and Batrachians in Tunisia. Scientific exploration of Tunisia. National Imprimery, Paris, pp 32. Google Scholar
Mayr, E. 1963. Animal Species and Evolution, Cambridge, Harvard University Press, 797 pp. Crossref, Google Scholar
Millington, A. C., Drake, N. A., Townshend, J. R. G., Quarmby, N. A., Settle, J. J. and A. J. Reading. 1989. Monitoring salt playa dynamics using Thematic Mapper data, IEEE Transactions on Geoscience and Remote Sensing, 27(6): 754761. Crossref, Google Scholar
Mueller, R. L. 2006. Evolutionary rates, divergence dates, and the performance of mitochondrial genes in Bayesian phylogenetic analysis. Systematic Biology, 55(2): 289300. Crossref, Google Scholar
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583590. Google Scholar
Nouira, S. 2001. Conservation des zones humides littorales et des écosystèmes côtiers — Cap Bon. Coastal Protection and Planning Agency, Ministry of Environment and Sustainable Development, Tunisia, internal report, pp 33. Google Scholar
Posada, D. and K. A. Crandall. 2001. Intraspecific gene genealogies: trees grafting into networks. Trends in Ecology and Evolution, 16: 3745. Crossref, Google Scholar
Recuero, E., Iraola, A., Rubio, X., Machordom, A. and M. Garcìa-Parìs. 2007. Mitochondrial differentiation and biogeography of Hyla meridionalis (Anura: Hylidae): an unusual phylogeographical pattern. Journal of Biogeography, 34: 12071219. Crossref, Google Scholar
Rogers, A. R., and H. Harpending. 1992. Population growth curves in the distribution of pairwise genetic differences. Molecular Biology and Evolution, 9: 552569. Google Scholar
Romdhane, M. S. and H. Missaoui. 2001. Conservation des Zones Humides Littorales et des Ecosystèmes côtiers du Cap-Bon. Coastal Protection and Planning Agency, Ministry of Environment and Sustainable Development, Tunisia, internal report, pp 24. Google Scholar
Rozas, J., Sánchez-del Barrio, J. C., Messeguer, X. and R. Rozas. 2003. DNASP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics, 19: 24962497. Crossref, Google Scholar
Sambrook, J., Fritsch, E. F. and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2ème édition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor N.Y., 1659 pp. Google Scholar
Sanmartín, I. 2003. Dispersal vs. vicariance in the Mediterranean: historical biogeography of the Palearctic Pachydeminae (Coleoptera, Scarabaedoidea). Journal of Biogeography, 30: 18831897. Crossref, Google Scholar
San Mauro, D., Garcia-Paris, M. and R. Zardoya. 2004. Phylogenetic relationships of discoglossid frogs (Amphibia: Anura: Discoglossidae) based on complete mitochondrial genomes and nuclear genes. Gene, 343: 357366. Crossref, Google Scholar
San Mauro, D., Vences, M., Alcobendas, M., Zardoya, R. and A. Meyer. 2005. Initial diversification of living amphibians predated the breakup of Pangaea. American Naturalist, 165: 590599. Crossref, Google Scholar
Sicilia, A., Marrone, F., Sindaco, R., Turki, S. and Arculeo, M. 2007. Contribute to the knowledge of Tunisian amphibians: notes on distribution, habitat features and phenology. Proceedings of the Fifth Symposium for European Freshwater Sciences (SEFS5), Palermo, 8–13 July 2007, p. 249. Google Scholar
Smith, M. A. and D. M. Green. 2005. Are all amphibian populations metapopulations? Dispersal and the metapopulation paradigm in amphibian ecology. Ecography, 28: 110128 Crossref, Google Scholar
Tajima, F. 1989. Statistical methods for testing the neutral mutation hypothesis for DNA polymorphism. Genetics, 123: 585595. Google Scholar
Templeton, A. R. 1998. Nested clade analyses of phylogeographic data: testing hypotheses about gene fow and population history. Molecular Ecology, 7: 381397. Crossref, Google Scholar
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and D. G. Higgins. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24: 48764882. Crossref, Google Scholar
Tryjanowski, P., Rybacki, M. and T. Sparks. 2003. Changes in the first spawning dates of common frogs and common toads in western Poland in 1978–2002. Annales Zoologici Fennici, 40: 459464. Google Scholar
Vences, M. and D. B. Wake. 2007. Speciation, species boundaries and phylogeography of amphibians. In Amphibian Biology Volume 7. Edited by: Heatwole H, Tyler M. Chipping Norton, Australia, Surrey Beatty & Sons: 26132671. Google Scholar
Walther, G. R., Post, E., Convey, P., Menzel, A., Parmesan, C., Beebee, T. J. C., Fromentin, J. M., Hoegh-Guldberg, O. and F. Bairlein. 2002. Ecological responses to recent climate change. Nature, 416: 389395. Crossref, Google Scholar
Zangari, F., Cimmaruta, R. and G. Nascetti. 2006. Genetic relationships of the western Mediterranean painted frogs based on allozymes and mitochondrial markers: evolutionary and taxonomic inferences (Amphibia, Anura, Discoglossidae). Biological Journal of the Linnean Society, 87: 515536. Crossref, Google Scholar

Cited by

Susanne Hauswaldt, Ralf Hendrix, David Donaire, Aziza Lansari, El Hassan El Mouden, Abdellah Bouazza, Tahar Slimani, Ulrich Joger, Miguel Vences. (2015) The Atlas Massif separates a northern and a southern mitochondrial haplotype group of North African water frogs Pelophylax saharicus(Anura: Ranidae) in Morocco. Amphibia-Reptilia 36:4, 437-443.
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Violaine Nicolas, Abderrahmane Mataame, Pierre-André Crochet, Philippe Geniez, Annemarie Ohler. (2015) Phylogeographic patterns in North African water frog Pelophylax saharicus (Anura: Ranidae). Journal of Zoological Systematics and Evolutionary Research 53:3, 239-248.
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