In the Middle-Atlantic region, the distribution, abundance, and habitat associations of fireflies in the genus Photuris are poorly understood. Six species in Photuris are recorded from Delaware. Ten years (1998–2008) of periodic survey work has yielded three new state records and contributed valuable information regarding species abundance and habitat affiliations in this region. Results from that survey are summarized and compared to previously published Delaware records. This is the first concerted effort to report the conservation status and the associated habitat and natural community types of Photuris fireflies from the Middle-Atlantic region.