The mosquito larvicidal and pupicidal potential of the silicone-based monomolecular film Aquatain® Mosquito Formula (AMF), was investigated in laboratory trials against Aedes aegypti and Culex quinquefasciatus. Larval and pupal mortality was investigated in separate trials. After 48 h of exposure, mean mortality of Cx. quinquefasciatus 4^th instars was 94.6%, significantly greater than the mean mortality rate of 33.6% for Ae. aegypti. After 180 min of exposure, 100% mortality of pupae was recorded for both species. Mean larval and pupal mortality rates for both species were significantly greater than mortality rates in untreated controls. The results indicate that AMF holds potential for mosquito control, especially in urban water-holding structures that are becoming increasingly popular in response to water conservation. Nontarget impacts must be investigated before this product can be considered for natural wetlands.