Outbreaks of lipophilic toxins have been recorded in the north Atlantic coast of Morocco since 1999, but are rare in the Mediterranean coast. Samples of mussels from the Atlantic coast where toxicity was detected by mouse bioassay were stored for further research. Chemical analysis by LC-MS conducted in mussels harvested from this region showed, in addition to the presence of okadaic acid (OA) and dinophysistoxin-2 (DTX2), the presence of azaspiracid-2 (AZA2) as the dominant form of the azaspiracid's (AZAs) family, followed by AZA1 (13% to 26%). AZA3 was rarely detected, and maximal concentrations found were between 3% and 8% of total AZA1/3. The presence of AZA2 and AZA1 was confirmed by mass spectra. Time series corresponding to the summer of 2004 and 2005 showed maximal concentration of AZAs appeared in July in both years. Correlation with occurrence of OA and DTX2, showed both toxin families could appear simultaneously in Moroccan mussels, but maximal concentrations found were always separated in time. This is the first report of azaspiracids in Morocco (NW Africa) and the first report outside of European coastlines.