This paper highlights André Michaux's journey in Canada at the end of the Eighteenth Century. On June 7, 1792, André Michaux left New York City for Canada. He sailed up the Hudson River and pursued his itinerary to Lower Canada. Michaux's intention was to reach Hudson Bay. His scientific expedition consisted of analyzing the geographical distribution of North American plants and determining their habitats. From the Hudson River to the Rivière Mistassini (Canada), he described the natural beauty of the Appalachian Mountains (United States), lakes and rivers; he traveled through the Laurentian Mountains (Canada); he continuously observed and collected plants on his scientific expedition; and he identified animals that he specifically noticed between Lac St-Jean and Lac Mistassini. These principal aspects of his journey in Canada from June to November 1792 were the prime concern for this study.