Zoological Science

Published by: Zoological Society of Japan



Zoological Science 14(5):785-789. 1997
doi: http://dx.doi.org/10.2108/zsj.14.785

Molecular Cloning of a Moil-Inhibiting Hormone cDNA from the Kuruma Prawn Penaeus japonicus

Tsuyoshi Ohira1, Toshiki Watanabe2,*, Hiromichi Nagasawa2, and Katsumi Aida1

1Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Tokyo 113, Japan

2Laboratory of Molecular Biology of Marine Organisms, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo 164, Japan

* Corresponding author: Tel. +81-3-5351-6534; FAX. +81-3-5351-6488

Abstract

The crustacean molt-inhibiting hormone (MIH) is released from the X-organ sinus gland complex and suppresses ecdysteroid synthesis by the Y-organ. In the present study, we have isolated a cDNA which encodes a MiH (Pej-SGP-IV) of the kuruma prawn Penaeus japonicus in order to study its expression and characterize the structure of its precursor. A cDNA fragment was isolated using RT-PCR with two degenerate oligonucleotide primers that were designed based on the peptide sequence of Pej-SGP-IV, and this fragment was used as a probe to screen an eyestalk cDNA library. In a positive cDNA clone (814 base pairs (bp)), an open reading frame of 315 bp was found; the conceptually translated protein consists of a putative signal peptide (28 residues) and Pej-SGP-IV (77 residues). In Northern blot analysis using a cDNA probe, specific hybridization to a transcript of 0.95 kb was seen in RNA extracted from the eyestalk but not from hepatopancreas, abdominal muscle, brain, thoracic ganglia or abdominal ganglia. The level of the Pej-SGP-IV mRNA in the eyestalk did not change significantly during the molt cycle.

Received: April 10, 1997; Accepted: June 24, 1997



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