Reports of the giant anteater (Myrmecophaga tridactyla) in Central America, and specifically in Honduras, are sporadic and typically of just one individual. During a 3.5-year (2001–2005) Honduran Program of Biological Monitoring, four new reports of the giant anteater were made in protected areas located in the Honduran Mosquitia in the departments of Gracias a Dios and Olancho with the most sightings made in the proposed protected area of Rus Rus. Three additional reports independent of the Honduran Program of Biological Monitoring are documented herein as well; these reports also are from the protected area of Rus Rus. We conclude that the giant anteater probably has been extirpated from the Caribbean region of Honduras, but remains present in the Honduran Mosquitia in the departments of Gracias a Dios and Olancho.