We report on the natural history of Ambystoma altamirani from the Arroyo Los Axolotes, State of Mexico. Ambystoma altamirani used stream sites that hold a greater volume of water, higher dissolved oxygen levels, and faster moving water compared with those without A. altamirani. They used sites with grassy vegetation, and mud and sandy substrate. Males were larger than females. We observed three egg masses in June 2015 (mean = 21 eggs). Metamorphosis occurred between 62 and 70 mm snout–vent length. Our results indicate that Arroyo Los Axolotes must be managed to maintain conditions and water flow amenable to the persistence of A. altamirani.