We measured and compared the circular dichroism (CD) spectra and secondary structures of histone proteins H2A, H2B and their variants extracted from X-irradiated and unirradiated human HeLa cells. Compared to unirradiated cells, a relative increase in α-helix structure and decrease in other secondary structures was observed in X-irradiated cells. These structural alterations persisted for at least 24 h, which is substantially longer than the 2 h generally known to be required for DNA double-strand break repair.
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1 November 2015
Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours
Yudai Izumi,
Satoshi Yamamoto,
Kentaro Fujii,
Akinari Yokoya
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Radiation Research
Vol. 184 • No. 5
November 2015
Vol. 184 • No. 5
November 2015