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Radiation Research

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Radiation Research 169(6):633-638. 2008
doi: http://dx.doi.org/10.1667/RR1190.1

Resveratrol Reduces Radiation-Induced Chromosome Aberration Frequencies in Mouse Bone Marrow Cells

Ronald E. Carsten^a ^b, Annette M. Bachand^b, Susan M. Bailey^a ^b, and Robert L. Ullrich¹ ^a ^b

^aCell and Molecular Biology, Colorado State University, Fort Collins, Colorado

^bDepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado

¹⁰¹¹ Address for correspondence: 433 MRB Building, ERHS Dept Campus Delivery Code 1618, Colorado State University, Fort Collins, CO 80523; bullrich@colostate.edu

Abstract

Carsten, R. E., Bachand, A. M., Bailey, S. M. and Ullrich, R. L. Resveratrol Reduces Radiation-Induced Chromosome Aberration Frequency in Mouse Bone Marrow Cells. Radiat. Res. 169, 633–638 (2008).

Resveratrol, a polyphenol compound with reported antioxidant and anticarcinogenic effects, a wide range of molecular targets, and toxicity only at extreme doses, has received considerable attention. We evaluated the radioprotective effect of orally administered resveratrol on the frequencies of chromosome aberrations in irradiated mouse bone marrow cells. CBA/CaJ mice were divided into four groups: (1) no treatment, (2) resveratrol only, (3) radiation only, and (4) resveratrol and radiation. Resveratrol treatment (100 mg/kg daily) was initiated 2 days prior to irradiation. Bone marrow was then harvested at 1 and 30 days after a single dose of 3 Gy whole-body γ radiation. A statistically significant (P < 0.05) reduction in the mean total chromosome aberration frequency per metaphase at both times postirradiation in the resveratrol and radiation group compared to the radiation-only group was observed. This study is the first to demonstrate that resveratrol has radioprotective effects in vivo. These results support the use of resveratrol as a radioprotector with the potential for widespread application.

Received: August 9, 2007; Accepted: January 10, 2008

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FIG. 1. Photomicrographs of metaphase bone marrow cells 1 day postirradiation. Panel A: radiation only, panel B: radiation + resveratrol, panel C: no treatment, and panel D: resveratrol only. Arrows indicate chromosome fragments (f) and Robertsonian translocations (r)

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FIG. 2. Total mean chromosome aberrations per bone marrow metaphase cell ± SE day 1 after 3 Gy whole-body irradiation. Statistically significant differences at the P < 0.05 level were found when comparing the no treatment (No Tx) and radiation (RAD) groups and the radiation (RAD) and resveratrol + radiation (RES + RAD) groups for mean total aberrations

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FIG. 3. Total mean chromosome aberrations per bone marrow metaphase cell ± SE 30 days postirradiation. Statistically significant differences at the P < 0.05 level were found when comparing the no treatment (No Tx) and radiation (RAD) groups and the radiation (RAD) and resveratrol + radiation (RES + RAD) groups for mean total aberrations

TABLE 1 Mean Chromosome Aberrations per Bone Marrow Metaphase Cell Observed in Each Mouse Group at Each Time

TABLE 2 Statistical Comparisons between Mouse Treatment Groups and Aberration Categories at Each Time

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