This study was carried out in the regions where handcrafts such as carpet and rug weaving are common in Turkey. In the regions where natural dyes are used, 123 plant species belonging to 50 families were identified as sources of natural dyes. In natural dye production, different parts of the plant or the whole aboveground plant is used. Ten different colors are gained from the plants. Sometimes the same color can be obtained from different plants. By mixing different plants, it is possible to produce various colors. We also identified more than 12 natural and more than eight chemical assistant substances (mordant) that are used in the regions to bind dye to fibers, to maintain the strongness and brightness of the colors, and to obtain various colors.