The use of HMDS (hexamethyldisilazane) to Replace Critical Point Drying (CPD) in the Preparation of Tardigrades for SEM (Scanning Electron Microscope) Imaging

Shawn Shively; William R. Miller

doi:10.1660/062.112.0407

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Shawn Shively,¹ William R. Miller¹

¹Department of Biology, Baker University, Baldwin City, Kansas 66006 William.Miller@bakeru.edu

Abstract

HMDS (hexamethyldisilazane) may be used to replace the critical point drying step in sample preparation of tardigrades for SEM imaging. Previous procedures required a Critical Point Drying apparatus or machine to achieve the temperature/pressure combination to completely dehydrate a small specimen for SEM work. Now a simple bath in HMDS brings the specimens to the same state of dehydration without special equipment. Results are equal to the previous method.

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Shawn Shively and William R. Miller "The use of HMDS (hexamethyldisilazane) to Replace Critical Point Drying (CPD) in the Preparation of Tardigrades for SEM (Scanning Electron Microscope) Imaging," Transactions of the Kansas Academy of Science 112(4), 198-200, (1 September 2009). https://doi.org/10.1660/062.112.0407

Published: 1 September 2009

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