Microsatellite multiplexing is a useful technique that minimizes the time, reagents, and cost associated with genetic studies in fisheries biology. Striped Bass is an important sport and aquaculture species commonly stocked throughout the United States. We have developed three multiplexed panels that collectively incorporate twelve different established microsatellite loci. All loci were tested for Hardy-Weinberg equilibrium, linkage disequilibrium, Mendelian inheritance, and null alleles in two populations. Loci were comparably polymorphic in two river systems with similar allele size ranges observed; therefore, these multiplexed panels should be useful for genetic population studies of Striped Bass both within and between disparate geographic distributions.