Populations of Ambystoma barbouri (Streamside Salamander) have recently been identified in the Central Basin of middle Tennessee. Little is known regarding the distribution, natural history, and health of populations in Tennessee, resulting in the salamander being “deemed in need of management” by state agencies. Here we provide information regarding reproduction for the only known extant populations in Rutherford County, TN. In February 2005, fifty-one egg masses from three first-order streams were digitally photographed and analyzed to determine size of egg mass, stage of development, ovum diameter, and embryo length. Number of eggs per mass, size of ova, and size of embryos were comparable to that reported in the literature. Egg masses varied noticeably in stage of embryonic development; we estimate that oviposition in 2005 occurred between early December and early February. Eurycea cirrigera (Southern Two-lined Salamanders) oviposited in the same first-order streams and during the same time period as Streamside Salamander. Continued deforestation and land development associated with urban sprawl from the city of Murfreesboro threaten existing populations of Streamside Salamander. The information in this study can be used to provide a basis for developing conservation plans for the Streamside Salamander in middle Tennessee and for scheduling construction activities such that they least affect breeding activities.