Four cercopitheciid primate species at an academic institution research facility within the United States were found infected with an Enterobius species that was morphologically and genetically distinct from all currently known species of pinworms and is herein described as Enterobius shriveri n. sp. Phylogenetic analysis is provided for E. shriveri based on analysis of a partial 18S ribosomal RNA gene (rDNA) and a partial mitochondrial cytochrome c oxidase I (Cox1) gene. Three of the 15 infected hosts were also co-infected with Enterobius macaci Yen, 1973. Electron microscopy and light microscopy images of both male and female E. shriveri and E. macaci specimens are provided, as are additional morphologic and morphometric data for E. macaci, both of which indicate differences between Asiatic mainland and Japanese island isolate forms.