For the first time in Tajikistan, South American tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) was detected in greenhouses and open field environments in Khatlon region and the region of Republican Subordination. Leaf damage, although lower at the beginning of the season, gradually rose to 15–30% in surveyed sites. Fruit damage also gradually increased during the season. Fruit damage was as high as 20% in the locations surveyed, with the exception of Guliston and Ghayrat Jamoats, which had very low fruit damage. Tuta absoluta is becoming established in Tajikistan. Suitable integrated pest management strategies are needed to reduce the incidence of this invasive pest species.