Feeding by Frankliniella (Thysanoptera: Thripidae) thrips causes economic damage to strawberry (Fragaria ananassa Duchesne; Rosales: Rosaceae) crops in Florida and in other production regions worldwide. Resistance to spinosyn insecticides, particularly in Frankliniella occidentalis (Pergande), is a major concern for strawberry and other crops. Experiments were carried out in 2014 and 2015 to evaluate the effect of 6 insecticide programs on the numbers and species composition of thrips attacking strawberry on a season-long basis in Florida. Five insecticide programs included spinetoram applied once, twice, or 3 times in the rotation, alternated with acetamiprid, cyantraniliprole (Cyazypyr®), novaluron, sulfoxaflor, and/or tolfenpyrad. Also included in the treatments were bifenthrin and a non-treated check. Thrips densities were sampled weekly in flowers, and in both flowers and fruits in 2015, 2 d after treatment applications. The primary thrips species recovered from strawberry flowers and fruit was Frankliniella bispinosa Morgan. Other species included F. occidentalis, Frankliniella schultzei (Trybom), Scirtothrips dorsalis Hood, Scolothrips sp., Thrips spp. (all Thripidae), and Haplothrips gowdeyi (Franklin) (Phlaeothripidae), Frankliniella bispinosa was controlled by all insecticide programs. Numbers of F. occidentalis thrips were not reduced by any spinetoram-based rotation relative to the control in either year. Repeated applications of bifenthrin increased numbers of F. occidentalis thrips relative to the control each year, and increased numbers of F. schultzei thrips relative to the control in 2014. The thrips predator Orius sp. (Hemiptera: Anthocoridae) was not observed in the bifenthrin treatment and was rare in other treatments. Insecticide rotations in Florida strawberry appear to shift the species composition from F. bispinosa to F. occidentalis and other insecticide-tolerant species including F. schultzei. Thrips damage to strawberries may be due to the species that is least susceptible to control rather than the species that is most abundant early in the cropping season. However, the relative importance of various stages or species has yet to be critically determined.