A 3-year-old female peregrine falcon-gyrfalcon hybrid (Falco peregrinus × Falco rusticolus) was presented for evaluation of a lesion below the right eye. Initial examination revealed an abraded, crusty lesion on the right lower eyelid that was believed to be consistent with trauma and a secondary bacterial infection. Despite initial diagnostic procedures and treatment, the condition progressed to severe blepharitis and dermatitis involving the upper and lower eyelids of both eyes and the head. Septated fungal hyphae were identified by histopathologic analysis in specimens obtained from the lesions and an Aspergillus species was cultured. The blepharitis and dermatitis were treated successfully with oral itraconazole (15 mg/kg q12h) and topical miconazole cream, applied twice daily.