Three-Dimensional Imaging of Monogenoidean Sclerites by Laser Scanning Confocal Fluorescence Microscopy

Paolo Galli; Giovanni Strona; Anna Maria Villa; Francesca Benzoni; Stefani Fabrizio; Silvia Maria Doglia; Delane C. Kritsky

doi:10.1645/GE-3544RN.1

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Paolo Galli,¹ Giovanni Strona,¹ Anna Maria Villa,¹ Francesca Benzoni,¹ Stefani Fabrizio,¹ Silvia Maria Doglia,¹ Delane C. Kritsky^2,*

¹aDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2,
²bDepartment of Health and Nutrition Sciences, Campus Box 8090, Idaho State University, Pocatello, Id

^** corresponding author. kritdela@isu.edu

Abstract

A nondestructive protocol for preparing specimens of Monogenoidea for both α-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy.

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Paolo Galli, Giovanni Strona, Anna Maria Villa, Francesca Benzoni, Stefani Fabrizio, Silvia Maria Doglia, and Delane C. Kritsky "Three-Dimensional Imaging of Monogenoidean Sclerites by Laser Scanning Confocal Fluorescence Microscopy," Journal of Parasitology 92(2), 395-399, (1 April 2006). https://doi.org/10.1645/GE-3544RN.1

Published: 1 April 2006

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