Journal of Zoo and Wildlife Medicine
Published by: American Association of Zoo Veterinarians
Journal of Zoo and Wildlife Medicine 38(2):292-299. 2007
doi: 10.1638/1042-7260(2007)038[0292:SCEICE]2.0.CO;2
SYSTEMIC CALICIVIRUS EPIDEMIC IN CAPTIVE EXOTIC FELIDS







101From the Potter Park Zoo, 1301 South Pennsylvania, Lansing, Michigan 48912, USA (Harrison); Michigan State University College of Veterinary Medicine, East Lansing, Michigan 48824, USA (Harrison, Kruger, Sikarskie); and the Diagnostic Center for Population and Animal Health, Michigan State University, 4125 Beaumont Road, East Lansing, Michigan 48910, USA (Kiupel, Maes, Mullaney, Wise). Correspondence should be directed to Dr. Harrison (tharriso@ci.lansing.mi.us)
Abstract
A 5-day-old, mother-raised, Amur tiger cub (Panthera tigris altaica) presented with tongue ulcerations. Identical lesions appeared and progressed to sloughing of the tongue in the three littermates of this cub the following day. The lesions progressed in all cubs to include sloughing of the carpal, tarsal, metacarpal, and metatarsal foot pad epithelium. Oral ulcerations were also noted in adult African lions (Panthera leo) and Amur tigers (Panthera tigris altaica), but not in two adult snow leopards (Panthera uncia) housed in the same building. All adult cats had been previously vaccinated for common feline diseases including feline calicivirus (FCV). Detection of FCV RNA in oral secretions by a real-time reverse transcription polymerase chain reaction assay (RRT-PCR) confirmed FCV infection in the tiger cubs and one lion. A male lion and a male tiger cub died during the disease outbreak. RRT-PCR confirmed FCV in multiple tissues in both of these animals. A stray cat live-trapped outside the feline building during the epidemic was found to be positive for FCV by virus isolation and was thought to be the source of infection.
Received: April 4, 2006
LITERATURE CITED
,
,
,
, and
. 2004. Siberian tiger (Panthera tigris altaica) species survival plan update. Proc. Am. Assoc. Zoo Vet. Annu. Meet. 2004:321–325.
,
,
, and
. 2006. Survival on uncommon fomites of feline calicivirus, a surrogate of noroviruses. Am. J. Infect. Control 34:41–43. CrossRef, PubMed
,
,
,
, and
. 1999. Inactivation of feline calicivirus, a Norwalk virus surrogate. J. Hosp. Infect. 41:51–57. CrossRef, PubMed, CSA
,
,
,
,
,
, and
. 2006. Persistence of caliciviruses on environmental surfaces and their transfer to food. Int. J. Food Microbiol. 108:84–91. CrossRef, PubMed
,
,
,
,
, and
. 2004. Inactivation of caliciviruses. Appl. Environ. Microbiol. 70:4538–4543. CrossRef, PubMed
,
,
,
, and
. 2006. Virulent systemic feline calicivirus infection: local cytokine modulation and contribution of viral mutants. J. Feline Med. Surg. 8:55–61. CrossRef, PubMed
,
,
,
,
,
, and
. 2005. Treatment of malignant lymphoma in an African lion (Panthera leo). J. Zoo and Wildl. Med. 38:333–336. . CrossRef
,
,
,
,
,
,
, and
. 1996. Prevalence of antibodies to feline parvovirus, calicivirus, herpesvirus, coronavirus, and immunodeficiency virus and of feline leukemia virus antigen and the interrelationship of these viral infections in free-ranging lions in east Africa. Clin. Diagn. Lab. Immunol. 3:554–62. PubMed, CSA
,
,
,
, and
. 2004. An outbreak of virulent systemic feline calicivirus disease. J. Am. Vet. Med. Assoc. 224:241–249. CrossRef, PubMed
and
. 2003. Update on feline calicivirus: new trends. Vet. Clin. North Am. Small Anim. Pract. 33:759–772. CrossRef, PubMed, CSA
,
,
,
,
, and
. 1997. A strain of calicivirus isolated from lions with vesicular lesions on tongue and snout. New Microbiol. 20:141–148. PubMed, CSA
,
,
, and
. 2002. Use of serologic tests to predict resistance to feline herpesvirus 1, feline calicivirus, and feline parvovirus infection in cats. J. Am. Vet. Med. Assoc. 220:38–42. CrossRef, PubMed, CSA
,
,
, and
. 2004. Duration of serologic response to three viral antigens in cats. J. Am. Vet. Med. Assoc. 224:61–66. CrossRef, PubMed
,
, and
. 1973. Feline picornavirus infection: the in vivo carrier state. Vet. Rec. 92:224–229. PubMed
,
,
,
,
,
, and
. 2001. Endemic infection of a cat colony with a feline calicivirus closely related to an isolate used in live attenuated vaccines. Vaccine 19:4358–4362. CrossRef, PubMed, CSA
,
,
,
,
,
,
, and
. 1993. Seroprevalence of infectious disease agents in free-ranging Florida panthers (Felis concolor coryi). J. Wildl. Dis. 29:36–49. PubMed, CSA
,
,
,
, and
. 2004. Evaluation of a p30 gene-based real-time reverse transcriptase polymerase chain reaction assay for detection of feline caliciviruses. J. Vet. Intern. Med. 18:135–138. CrossRef, PubMed
,
,
, and
. 2003. An epizootic of highly virulent feline calicivirus disease in a hospital setting in New England. J. Feline Med. and Surg. 5:217–226. CrossRef, PubMed
and
. 1999. Long-term immunity in cats vaccinated with an inactivated trivalent vaccine. Am. J. Vet. Res. 60:652–658. PubMed, CSA
,
, and
. 1998. Detection and strain differentiation of feline calicivirus in conjunctival swabs by RT-PCR of the hypervariable region of the capsid protein gene. Arch. Virol. 143:1321–1334. CrossRef, PubMed, CSA
,
,
,
, and
. 2002. Tiger SSP Population Status, International Programs, Contraception and Vaccination Recommendations. Tiger SSP Report. Pp. 1-10.
and
. 1977. The pathology and sites of persistence associated with three different strains of feline calicivirus. Res. Vet. Sci. 23:7–14. PubMed
,
, and
. 1996. Diagnosis and therapy of suspected calicivirus infection in tigers and lions. Chin. J Vet. Sci. Technol. 26:37–38.
Figure 1.
Tongue from tiger cub that died on day 12 of a virulent systemic feline calicivirus infection. Note the necrosis and sloughed tissue from the distal tip of the tongue and the mucosal ulceration of the base of the tongue.
Table 1.
Serum neutralization titers, day clinical signs were observed, real-time reverse transcription polymerase chain reaction assay (RRT-PCR) results, and virus isolation results for feline calicivirus in exotic felids housed in feline house that experienced a calicivirus epidemic. Days are reported in relation to day 1, the first day that clinical signs were noted in the first case of the outbreak.


