Annals of the Entomological Society of America

Published by: Entomological Society of America



Annals of the Entomological Society of America 99(6):1028-1033. 2006
doi: 10.1603/0013-8746(2006)99[1028:ANKTPS]2.0.CO;2

A Nondichotomous Key to Protist Species Identification of Reticulitermes (Isoptera: Rhinotermitidae)

J. L. Lewis1 and B. T. Forschler

Department of Entomology, 413 Biological Sciences Building, University of Georgia, Athens, GA 30602

1Corresponding author, .

Abstract

A key was developed using morphological and behavioral characters to identify nine genera and 13 species of protists found in the hindgut of three Reticulitermes species— Reticulitermes flavipes (Kollar), Reticulitermes virginicus (Banks), and Reticulitermes hageni Banks—by using the online IDnature guides by Discover Life. There are seven characters and 13 taxa, each attached to species descriptions, digital stills, or movies to aid in protist species identification. We chose characters for protist species identification that were easy to observe with live samples and a light microscope at 400× magnification. All 11 protists from R. flavipes and nine each in R. virginicus and R. hageni were recognized using original and revised species descriptions. This was the first report of the protist genera Trichomitus from both R. virginicus and R. hageni.

Received: March 23, 2006; Accepted: July 5, 2006





Host Species Protist Community

Reticulitermes flavipes (Fig. 1).

Reticulitermes virginicus (Fig. 2).

Reticulitermes hageni (Fig. 3).



Placement of Flagella

Anterior region only (Fig. 1d, f, and h; Fig. 2a, e, and i; Fig. 3f and g).

Anterior and posterior region, including recurrent flagella (Fig. 1h, Fig. 2i, Fig. 3g).

Flagellar cords (Fig. 1a, b, and j; Fig. 2g and h; Fig. 3a and b).

Posterior region only (Fig. 1a, b, j, and k; Fig. 2g and h; Fig. 3a and b).

Spiraling flagellar rows extend almost to the posterior end (Fig. 1c and g; Fig. 2b and c; Fig. 3d and i).

Spiraling flagellar rows extend to posterior end (Fig. 1i, Fig. 2d, Fig. 3e).

Undulating membrane (Fig. 1e, Fig. 2f, Fig. 3c).



Indicator Protist Species Used in Termite Identification

Reticulitermes flavipes (Fig. 1b).

Reticulitermes virginicus (Fig. 2a).

Reticulitermes hageni (Fig. 3b).

Original and revised protist species descriptions (Leidy 1877, 1881; Grassi 1879, 1892, 1917; Koidzumi 1916, 1917; Dubosq and Grassé 1924, 1928; Kirby 1924, Powell 1928, Brown 1930a,b, 1931; Boykin et al. 1986; Lewis and Forschler 2004b) following the terminology of Koidzumi (1921) and Kudo (1966)



References Cited

Boykin, M. S., L. Stockert, H. E. Buhse Jr, and H. E. Smith-Somerville. Trichomitus trypanoides (Trichomonadida) from the termite Reticulitermes flavipes. II. Fine structure and identification of the cloned flagellate. Trans. Am. Microsc. Soc. 105:1986. 223238. CrossRef
Brown, V. E. On the morphology of Spirotrichonympha with a description of two new species from Reticulitermes hageni Banks. Arch. Prot. 70:1930a. 517530.
Brown, V. E. Hypermastigote flagellates from the termite Reticulitermes: Torquenympha octoplus gen. nov., sp. nov., and two new species of Microjoenia. Univ. Calif. Publ. Zool. 36:1930b. 6780.
Brown, G. V. E. The morphology of Spironympha, with a description of a new species from Reticulitermes hesperus Banks. J. Morph. Physiol. 51:1931. 291307.
Calkins, G. N. The biology of the Protozoa. 1926. Lea and Febiger. Philadelphia, PA.
Cook, T. J. Community structure and assemblage interaction among the symbiotic flagellates of subterranean termites in the genus Reticulitermes (Holmgren). Ph.D. dissertation. 1996. Texas A&M University. College Station, TX.
Dropkin, V. H. The ecology of the termite gut. Ph.D. dissertation. 1944. University of Chicago. Chicago, IL.
Dubosq, O. and P. P. Grassé. Notes sur les protistes parasites des termites de France. I. Trichomonas trypanoïdes n. sp. C. R. Seances Soc. Biol. Fil. 90:1924. 547550.
Dubosq, O. and P. P. Grassé. Notes sur les protistes parasites des termites de France. V. Les Spirotrichonympha et leur evolution. Arch. Zool. Exp. Gen. Notes Rev. 67:1928. 159178.
Grassi, B. Dei protozoi parassiti e specialmente di quelli che sono nell ‘uomo. Gazz. Med. Ital. Lomb. 39:1879. 445448.
Grassi, B. Conclusioni d’una memoria sulla società dei Termiti. Atti. Acad. Nat. R. Lincei. Sci. Fisiche 1:1892. 3336.
Grassi, B. Flagellati viventi nei Termiti. Mem. R. Acad. Lincei 12:1917. 331394.
Grube, S. and B. T. Forschler. Census of monogyne and polygyne laboratory colonies illuminates dynamics of population growth in Reticulitermes flavipes (Isoptera: Rhinotermitidae). Ann. Entomol. Soc. Am. 97:2004. 466475. CrossRef
Honigberg, B. M. Evolutionary and systematic relationships in the flagellate order Trichomonadida Kirby. J. Protozool. 10:1963. 2063. PubMed
Inoue, T., O. Kitade, T. Yoshimura, and I. Yamaoka. Symbiotic associations with protists. Abe, T. A., D. E. Bignell, and M. Higashi, editors. Termites: evolution, sociality, symbioses, and ecology. 2000. 275288.Kluwer Academic Publishers. Norwell, MA.
Kirby, H. Morphology and mitosis of Dinenympha fimbriata sp. nov. Univ. Calif. Publ. Zool. 26:1924. 199220.
Kirby, H. Flagellates of the genus Trichonympha in termites. Univ. Calif. Publ. Zool. 37:1932. 349476.
Kirby, H. Host-parasite relations in the distribution of protozoa in termites. Univ. Calif. Publ. Zool. 41:1937. 189212.
Kirby, H. Relationships between certain protozoa and other animals. Calkins, G. N. and F. M. Summers, editors. Protozoa in biological research. 3rd ed.1941. 8901008.Columbia University Press. New York.
Koidzumi, M. Studies on the Trichonymphids parasitic in the termites of Japan I. Annual Report of the Institute of Science, Government of Formosa 5:1916. 5583.
Koidzumi, M. Studies on the Trichonymphids parasitic in the termites of Japan II. Annual Report of the Institute of Science, Government of Formosa 6:1917. 93175.
Koidzumi, M. Studies on the intestinal protozoa found in the termites in Japan. Parasitology 13:1921. 235309.
Kudo, R. R. Protozoology. 5th ed.1966. Charles C Thomas Publisher. Springfield, IL.
Kudo, T., M. Ohkuma, S. Moriya, S. Noda, and K. Ohtoko. Molecular phylogenetic identification of the intestinal anaerobic microbial community in the hindgut of the termite, Reticulitermes speratus, without cultivation. Extremophiles 2:1998. 155161. CrossRef, PubMed
Lee, J. J., S. H. Hutner, and E. C. Bovee. An illustrated guide to the protozoa. 1985. 629. Society of Protozoologists. Lawrence, KS.
Leidy, J. On intestinal parasites of Termes flavipes. Proc. Acad. Nat. Sci., Phila. 29:1877. 146149.
Leidy, J. Parasites of the termites. J. Acad. Nat. Sci. Phila. 8:1881. 425450.
Lewis, J. L. and B. T. Forschler. Nitrogen-sparged media extends lifespan of symbiotic protists found in subterranean termites (Isoptera: Rhinotermitidae) providing more time for microscopic examination. Environ. Entomol. 33:2004a. 11451150. Abstract
Lewis, J. L. and B. T. Forschler. Protist communities from four castes and three species of Reticulitermes (Isoptera: Rhinotermitidae). Ann. Entomol. Soc. Am. 97:2004b. 12421251. CrossRef
Lewis, J. L. and B. T. Forschler. 2006. Discover life: protists found in termites. (http://discoverlife.org/20q?guide=Protists_Termites).
Mannesmann, R. Relationship between different wood species as a termite food source and the reproduction of termite symbionts. Z. Ang. Entomol. 72:1972. 116128.
Margulis, L., D. Chase, and R. Guerrero. Microbial Communities. BioScience 36:1986. 160170.
Nutting, W. L. Insecta: Isoptera. Dindal, D. L., editor. Soil biology guide. 1990. 9971032.Wiley. New York.
Ohkuma, M., S. Noda, and T. Kudo. Phylogenetic diversity of nitrogen fixation genes in the symbiotic microbial community in the gut of diverse termites. Appl. Environ. Microbiol. 65:1999. 49264934. PubMed, CSA
Ohkuma, M., K. Ohtoko, T. Iida, M. Tokura, S. Moriya, R. Usami, K. Horikoshi, and T. Kudo. Phylogenetic identification of Hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and parabasalian symbionts in the hindguts of termites. J. Eukaryot. Microbiol. 47:2000. 249259. Abstract, PubMed, CSA
Powell, W. N. On the morphology of Pyrsonympha with a description of three new species from Reticulitermes hesperus Bank. Univ. Calif. Publ. Zool. 31:1928. 179200.
Scheffrahn, R. H. and N-Y. Su. Keys to soldier and winged adult termites (Isoptera) of Florida. Fla. Entomol. 7777:1994. 460474.
Swezy, O. On a new trichomonad flagellate, Trichomitus parvus, form the intestine of amphibians. Univ. Calif. Publ. Zool. 16:1915. 8994.
Weesner, F. M. Termites of the Nearctic region. Krishna, K. and F. M. Weesner, editors. Biology of termites. vol. II. 1970. 447525.Academic. New York.
Yamin, M. A. Flagellates of the orders Trichomonadida Kirby, Oxymonadida Grassé, and Hypermastigida Grassi and Foà reported from lower termites (Isoptera families Mastotermitidae, Kalotermitidae, Hodotermitidae, Termopsidae, Rhinotermitidae, and Serritermitidae) and from the wood-feeding roach Cryptocercus (Dictyoptera: Cryptocercidae). Sociobiology 4:1979. 3119.
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