A new species of Passiflora (supersection Laurifolia, series Laurifoliae) from the Andean region of Colombia is described and illustrated using morphological descriptors analysis. This species is closely related to P. popenovii Killip and can be recognized mainly by its purple stem, leaf size (125–165 × 50–79 mm), biglandular petioles, pedicel length (8–10 mm), bracts light green, glandless, flowers length (28–30 mm), corona filaments in five series, minute-filiform inner filaments length (1–4 mm), fimbriate purplish operculum margin, staminal filaments length (6.8–7.1 mm), ovary glabrous, yellow mature fruits mottled with irregular white dots, lightly pubescent, and total soluble solids content in fruit juice (13.5%–14.3%). The newly identified species P. gustaviana grows on the slopes of high mountains between 1,900 and 2,309mabove sea level, with an annual mean temperature of 16.2°C. It is considered a new endemic species of Colombia and may be regarded as endangered (EN) because of its limited occurrence. This new species constitutes an important unexploited genetic resource useful for the improvement of cultivated Passiflora species.