An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the five largest beetle collections in Kentucky and from recent inventory work in state nature preserves revealed 31 species of the subfamily Cassidinae present in Kentucky, 13 of which are previously unreported for the state. Distribution maps and label data are presented for the 31 Kentucky species of the subfamily Cassidinae including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Anisostena ariadne (Newman), Anisostena nigrita (Olivier), Microrhopala excavata excavata (Olivier), Microrhopala rileyi S. Clark, Odontota horni J. Smith, Sumitrosis ancoroides (Schaeffer), Physonota unipunctata (Say), Cassida rubignosa Müller, Gratiana pallidula (Boheman), Erepsocassis rubella (Boheman), Jonthonota nigripes (Olivier), Opacinota bisignata (Boheman), and Strongylocassis atripes (LeConte).