Adult mosquito surveys of Saint Kitts and Nevis (SKN) were performed in the dry season (March 16–23, 2010) in Saint Kitts, and the rainy season (October 18–25, 2010) in SKN. Biogents (BG) Sentinel Traps were set with CO₂ and BG Lure in urban, rural, mangrove, and dry forest habitats. Mosquitoes were identified to species, and reverse transcription–polymerase chain reaction was performed on potential vector species for dengue virus (DENV), chikungunya virus (CHIKV), and West Nile virus (WNV). The most abundant species during both seasons in St. Kitts were Culex quinquefasciatus, Aedes taeniorhynchus, and Aedes aegypti. There were 3 new records for Saint Kitts: Aedes tortilis, Anopheles albimanus, and Culex nigripalpus. Traps were also set in Nevis. No mosquito pool tested positive for DENV, CHIKV, or WNV.